Publication: Reflexin A, a new indole alkaloid from Rauvolfia reflexa induces apoptosis against colon cancer cells
Date
2020
Authors
Fadaeinasab M.
Karimian H.
Omar H.
Taha H.
khorasani A.
Banisalam B.
Aziz Ketuly K.
Abdullah Z.
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor and Francis Ltd.
Abstract
One new indole alkaloid, reflexin A (1), and two known indoles, macusine B (2) and vinorine (3), were isolated from the bark of Rauvolfia reflexa. Their structures were elucidated by 1D and 2D NMR, UV, IR, and MS spectroscopic analyses. Compound 1 displayed anticancer activity against HCT-116 colon cancer cells with an IC50 value of 30.24 � 0.75 �M. The results implied that the newly isolated 1 induced apoptosis in HCT-116 cells, suggesting its possible role as an anticancer agent. In vivo acute toxicity study was performed on compound 1 to evaluate its safety profile. � 2019, � 2019 Informa UK Limited, trading as Taylor & Francis Group.
Description
antineoplastic agent; caspase 3; caspase 7; caspase 8; caspase 9; indole alkaloid; macusine; Rauvolfia reflexa extract; Rauwolfia extract; reflexin A; unclassified drug; vinorine; flavanone derivative; indole alkaloid; reflexin; acute toxicity; antineoplastic activity; apoptosis; Article; bark; cell invasion; cell migration; cell proliferation; colon cancer; controlled study; drug isolation; drug safety; drug structure; enzyme activation; G1 phase cell cycle checkpoint; HCT 116 cell line; human; human cell; IC50; Rauvolfia reflexa; Rauwolfia; apoptosis; chemical structure; colon tumor; Apoptosis; Colonic Neoplasms; Flavanones; Humans; Indole Alkaloids; Molecular Structure; Rauwolfia