Publication: Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner
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Date
2024
Authors
Leong Y.S.
Mokhtar M.H.H.
Ahmad Nazri N.A.
Abu Bakar M.H.
Zan M.S.D.
Arsad N.
Abdul P.M.
A. Bakar A.A.
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor and Francis Ltd.
Abstract
Carbon dots (CDs)-based fluorescence imaging with confocal reflectance effectively localises labelled structures with clear sample visualization. Yet, integrating these modalities into single-fibre cantilever-based endoscopic scanners poses challenges. Thus, we present an endoscopic scanner with a mixed-mode fibre cantilever for dual modalities imaging. The cantilever features two-attached fibre construction for multiple illumination and detection channels without additional optical components. The measured lateral and axial resolutions are 5.81 and 6.52 �m, respectively with field-of-view of 408 ? 488 �m. The fluorescence detection capability was first verified using fluorescent microspheres and CD-embedded polymer film, followed by confocal reflectance and CDs-based fluorescence imaging on labelled fibrous network and plant samples. The results demonstrate the complementary strengths of confocal reflectance, which extracts surface profiles, and CDs-based fluorescence, which captures fluorescence signals, leading to precise localization of fluorescence signals within the sample. Switching modality schemes at the scanner?s back-end enables implementation of different modalities. ? 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.
Description
Keywords
Nanocantilevers , Polymer films , Reflection , Semiconducting films , Single mode fibers , Carbon dots , Confocal reflectance , Endoscopic scanner , Fluorescence imaging , Fluorescence signals , Lissajous , Lissajous scanning , Mixed mode , Single fiber , Spatial localization , Fluorescence imaging