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Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications

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dc.contributor.authorSaiful Anuar N.F.en_US
dc.contributor.authorMisran H.en_US
dc.contributor.authorAhmad Salim M.en_US
dc.contributor.authorOthman S.Z.en_US
dc.contributor.authorid57189697186en_US
dc.contributor.authorid6506899840en_US
dc.contributor.authorid57189697454en_US
dc.contributor.authorid55548997100en_US
dc.date.accessioned2023-05-29T06:13:45Z
dc.date.available2023-05-29T06:13:45Z
dc.date.issued2016
dc.descriptionAlcohols; Enzyme immobilization; Glucose; Glucose oxidase; Glucose sensors; Palm oil; Radioactive waste vitrification; Spheres; Biosensing applications; Glucose biosensor; Glucose concentration; Glucose oxidases (GOx); Non-surfactant Template; Optimum temperature; Physical adsorption; Silica Sphere; Silicaen_US
dc.description.abstractIn this study, silica spheres (SiO2) nanomaterials were introduced as the support materials for glucose sensors. Glucose oxidase (GOx) was immobilized onto SiO2 spheres (SiO2/GOx) using physical adsorption (electrostatic interaction) method. The SiO2 spheres were synthesized in-house using a nonsurfactant template of palm oil derived fatty alcohols (PODFA). Based on FT-IR analyses, SiO2/GOx exhibited N-H absorbance at ca. 2990 cm-1 and O-H absorbance at ca. 3330 cm-1. One new absorbance peak was observed at ca. 2320 cm1 attributed to the bending vibration (?bend)of silane molecules (Si-H) obtained by the interaction of GOx and the surface silanols (Si-OH). UV-Vis analysis results exihibited the presence of a new broad peak for GOx/SiO2 sample at ca. 250 nm to 280 nm suggested to be assigned to H2O2, C=C and benzene ring from GOx. The optimum pH and optimum temperature for SiO2/GOx for GOx activity were ca. 7.0 and ca. 50 �C respectively. The increase of glucose concentration from 2mM to 5mM resulted to the increment of absorbance value for both GOx and SiO2/GOx suggesting succesful immobilization of GOx on SiO2 spheres. These materials were suitable for glucose detection at very small glucose concentrations particularly in salivary glucose detection. � 2016 Trans Tech Publications, Switzerland.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.4028/www.scientific.net/KEM.694.67
dc.identifier.epage71
dc.identifier.scopus2-s2.0-84974731264
dc.identifier.spage67
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84974731264&doi=10.4028%2fwww.scientific.net%2fKEM.694.67&partnerID=40&md5=763353a39bdf4245f0837858dce0a252
dc.identifier.urihttps://irepository.uniten.edu.my/handle/123456789/22962
dc.identifier.volume694
dc.publisherTrans Tech Publications Ltden_US
dc.sourceScopus
dc.sourcetitleKey Engineering Materials
dc.titleFacile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applicationsen_US
dc.typeConference Paperen_US
dspace.entity.typePublication
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