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Purification of lipase from Burkholderia metallica fermentation broth in a column chromatography using polymer impregnated resins

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dc.citedby1
dc.contributor.authorNg Z.J.en_US
dc.contributor.authorAbbasiliasi S.en_US
dc.contributor.authorYew Joon T.en_US
dc.contributor.authorNg H.S.en_US
dc.contributor.authorPhapugrangkul P.en_US
dc.contributor.authorTan J.S.en_US
dc.contributor.authorid57212007643en_US
dc.contributor.authorid46660902100en_US
dc.contributor.authorid58041560700en_US
dc.contributor.authorid48861933800en_US
dc.contributor.authorid37013726400en_US
dc.contributor.authorid35231307300en_US
dc.date.accessioned2024-10-14T03:21:30Z
dc.date.available2024-10-14T03:21:30Z
dc.date.issued2023
dc.description.abstractIn this work, porous glass beads grafted with polyethylene glycol (PEG) were used as an adsorbent to purify lipase from Burkholderia metallica in column chromatography. The purification parameters viz. salt stability, types and concentrations of PEG and salt, pH of the binding solution, and flow rate were studied to determine the performance of the purification system in an XK16/20 column. The crude lipase was mixed with different types and concentrations of salts 1�5% (w/w) (sodium citrate, potassium citrate, and sodium acetate) and subjected to the column containing the polymeric glass bead. One-variable-at-a-time experimentation revealed that 20% (w/w) PEG 6000 g/mol impregnated glass beads with a binding solution of 5% sodium citrate at pH 7.7, a flow rate of 1.0 mL/min and extraction time of 10 min resulted in the highest purification factor and recovery yield at 3.67 and 88%, respectively. The purified lipase has 55 ? 60 kDa molecular mass. The outcome of the study showed PEG could be applied to modify the inert glass beads into polymeric form, providing a biocompatible and mild separation condition for lipase. Thus, PEG could be successfully applied for the purification of lipase from B. metallica fermentation broth using column chromatography. � 2023 Taylor & Francis Group, LLC.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.1080/10826068.2022.2158468
dc.identifier.epage879
dc.identifier.issue7
dc.identifier.scopus2-s2.0-85145507120
dc.identifier.spage872
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85145507120&doi=10.1080%2f10826068.2022.2158468&partnerID=40&md5=181ab10dd15ab48abac1dd9465f09463
dc.identifier.urihttps://irepository.uniten.edu.my/handle/123456789/34658
dc.identifier.volume53
dc.pagecount7
dc.publisherTaylor and Francis Ltd.en_US
dc.sourceScopus
dc.sourcetitlePreparative Biochemistry and Biotechnology
dc.subjectBurkholderia metallica
dc.subjectcolumn chromatography
dc.subjectlipase
dc.subjectPEG grafted porous glass beads
dc.subjectpurification
dc.subjectBurkholderia metallica
dc.subjectChromatography
dc.subjectFermentation
dc.subjectHydrogen-Ion Concentration
dc.subjectLipase
dc.subjectPolyethylene Glycols
dc.subjectPolymers
dc.subjectmacrogol
dc.subjectpolymer
dc.subjecttriacylglycerol lipase
dc.subjectchemistry
dc.subjectchromatography
dc.subjectfermentation
dc.subjectpH
dc.titlePurification of lipase from Burkholderia metallica fermentation broth in a column chromatography using polymer impregnated resinsen_US
dc.typeArticleen_US
dspace.entity.typePublication
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