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Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system

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dc.citedby8
dc.contributor.authorKhoo K.S.en_US
dc.contributor.authorOoi C.W.en_US
dc.contributor.authorChew K.W.en_US
dc.contributor.authorChia S.R.en_US
dc.contributor.authorFoo S.C.en_US
dc.contributor.authorNg H.S.en_US
dc.contributor.authorShow P.L.en_US
dc.contributor.authorid57209198778en_US
dc.contributor.authorid57216184956en_US
dc.contributor.authorid57192980692en_US
dc.contributor.authorid57194081866en_US
dc.contributor.authorid56779646200en_US
dc.contributor.authorid48861933800en_US
dc.contributor.authorid47861451300en_US
dc.date.accessioned2023-05-29T09:37:43Z
dc.date.available2023-05-29T09:37:43Z
dc.date.issued2022
dc.descriptionAntioxidants; Flotation; Liquids; Molecular biology; Bio-compounds; Bioprocessing; Bubble separation; Chaetoceros calcitrans; Electropermeabilization; Electropermeabilization-assisted; Electroporation; Extraction systems; Fucoxanthin; Liquid biphasic flotation; Extraction; alcohol; copper; fucoxanthin; sodium chloride; fucoxanthin; xanthophyll; antioxidant activity; Article; biomass; bioprocess; Chaetoceros; Chaetoceros calcitrans; concentration (parameter); controlled study; diatom; electric potential; electroporation; extraction; flotation; flow rate; liquid biphasic flotation; nonhuman; partition coefficient; diatom; electroporation; microalga; Diatoms; Electroporation; Microalgae; Xanthophyllsen_US
dc.description.abstractElectropermeabilization-assisted liquid biphasic flotation (LBF) system is an emerging extraction system facilitated by the adsorptive bubble separation and the electroporation in an aqueous two-phase medium. This integrative extraction system is well suited for the direct recovery of intracellular biocompounds from cells without the needs of discrete steps of mid- or down-stream bioprocessing. The potential of electropermeabilization-assisted LBF system in bioseparation was demonstrated in this study by using the diatom Chaetoceros calcitrans (C. calcitrans) as the source of fucoxanthin, which is a promising antioxidant highly demanded by food and pharmaceutical industries. The extraction performances of LBF and electropermeabilization-assisted LBF system were comprehensively evaluated under the optimal operating conditions. Comparatively, the optimized LBF and electropermeabilization-assisted LBF systems achieved the satisfactory yields of fucoxanthin, i.e., 14.78 mg/g and 16.09 mg/g, respectively. The good recovery of fucoxanthin using electropermeabilization-assisted LBF system could be attributed to the higher release of fucoxanthin from the electrotreated C. calcitrans, allowing a higher partition of fucoxanthin to the top phase of LBF system (236.72 as compared to 152.15 from LBF system). In addition, the fucoxanthin extracted suing both methods exhibit satisfactory antioxidant activities. The application of electropermeabilization-assisted LBF system in the extraction of fucoxanthin is a greener and highly efficient bioprocessing route which can be prospectively extended to other biocompound extraction from microalgal sources. � 2022 Elsevier B.V.en_US
dc.description.natureFinalen_US
dc.identifier.ArtNo462915
dc.identifier.doi10.1016/j.chroma.2022.462915
dc.identifier.scopus2-s2.0-85125619442
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85125619442&doi=10.1016%2fj.chroma.2022.462915&partnerID=40&md5=81abad1f4bea39a0886ce906deaec91f
dc.identifier.urihttps://irepository.uniten.edu.my/handle/123456789/26911
dc.identifier.volume1668
dc.publisherElsevier B.V.en_US
dc.sourceScopus
dc.sourcetitleJournal of Chromatography A
dc.titleExtraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation systemen_US
dc.typeArticleen_US
dspace.entity.typePublication
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