Publication:
Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water

Simple item page
dc.citedby1
dc.contributor.authorThenarasu A.en_US
dc.contributor.authorChai M.K.en_US
dc.contributor.authorTan Y.H.en_US
dc.contributor.authorWong L.S.en_US
dc.contributor.authorRajamani R.en_US
dc.contributor.authorDjearamane S.en_US
dc.contributor.authorid57884752400en_US
dc.contributor.authorid24172523100en_US
dc.contributor.authorid57202000632en_US
dc.contributor.authorid55819849800en_US
dc.contributor.authorid57208159927en_US
dc.contributor.authorid57202375412en_US
dc.date.accessioned2025-03-03T07:43:04Z
dc.date.available2025-03-03T07:43:04Z
dc.date.issued2024
dc.description.abstractThe increased use of metallic nanoparticles has led to concern for environmental contamination and disruption in water quality. Therefore, effective screening of metallic nanoparticles is important for detecting metallic nanoparticles in aquatic environments. Biosensors offer several advantages, including high sensitivity to pollutants, short response time, energy efficiency, and low waste generation. In this study, a whole-cell biosensor was developed using microalga Chlorella vulgaris as a recognition element, and its fluorescence response was used as a measuring parameter for detecting the presence of titanium dioxide (TiO2) and silver (Ag) nanoparticles in water. The responses of C. vulgaris at the lag, exponential, and stationary phases to different concentrations of TiO2 and Ag nanoparticles were studied. The results showed that in TiO2 and Ag nanoparticles exposures, the highest fluorescence change (50-150%) was observed at the lag phase, whereas the lowest fluorescence change (40-75%) was observed at the stationary phase. A significant fluorescence change was observed in 15 min. The immobilized C. vulgaris under TiO2 and Ag nanoparticles exposures showed 30-180% higher fluorescence change than the negative control, indicating the potential of C. vulgaris as a biosensor for rapid detection of TiO2 and Ag nanoparticles in water. The mathematical modeling of the responses of C. vulgaris to TiO2 and Ag nanoparticles at 15 min of exposure with high R2 indicated that this biosensor is sensitive to the concentration tested (0.010-10.000 mg.L-1). Taken together, these results reveal that, for the first time, it is possible to detect TiO2 and Ag nanoparticles in water within a very short time using a microalgae-based biosensor. Moreover, no genetic engineering requirement makes this biosensor simple, economical, and free from the restriction on genetically modified microorganisms for environmental applications. ? 2024 Technoscience Publications. All rights reserved.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.46488/NEPT.2024.v23i02.030
dc.identifier.epage969
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85205026433
dc.identifier.spage959
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85205026433&doi=10.46488%2fNEPT.2024.v23i02.030&partnerID=40&md5=634a6f81e27cd72c06955bc7eb96c9f3
dc.identifier.urihttps://irepository.uniten.edu.my/handle/123456789/36558
dc.identifier.volume23
dc.pagecount10
dc.publisherTechnoscience Publicationsen_US
dc.relation.ispartofAll Open Access; Gold Open Access
dc.sourceScopus
dc.sourcetitleNature Environment and Pollution Technology
dc.subjectfluorescence
dc.subjectgenetic engineering
dc.subjectmicroalga
dc.subjectnanoparticle
dc.subjectsilver
dc.subjecttitanium
dc.subjectwater quality
dc.titleStudy of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Wateren_US
dc.typeArticleen_US
dspace.entity.typePublication
Files
Collections
SCOPUS