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Role of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase gene

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dc.citedby1
dc.contributor.authorUllah F.en_US
dc.contributor.authorMustafa G.en_US
dc.contributor.authorTariq Zahid M.en_US
dc.contributor.authorJamil I.en_US
dc.contributor.authorZaghum Abbas S.en_US
dc.contributor.authorJeon B.-H.en_US
dc.contributor.authorAlessa A.H.en_US
dc.contributor.authorRafatullah M.en_US
dc.contributor.authorid58400094300en_US
dc.contributor.authorid59423870200en_US
dc.contributor.authorid57215589901en_US
dc.contributor.authorid59153060700en_US
dc.contributor.authorid59152489700en_US
dc.contributor.authorid57653536800en_US
dc.contributor.authorid57981906100en_US
dc.contributor.authorid15050908200en_US
dc.date.accessioned2025-03-03T07:42:29Z
dc.date.available2025-03-03T07:42:29Z
dc.date.issued2024
dc.description.abstractBioremediation is a sustainable and worthy approach for remediating textile industrial wastewater, which poses severe extortions to human and environmental health due to its toxic, mutagenic, and carcinogenic nature. The present study employed Acinetobacter baumannii 1005 isolated from industrial wastewater to emphasize its degradation potential and to characterize the azoreductase (AZA) gene to assess and optimize the biodegradation of reactive blue 224 (RB-224) textile dye. A. baumannii showed significant decolorization against RB-224, resulting in a change in color. Under optimized conditions (pH 6, 37 �C, and 100 mg/L), A. baumannii exhibited 91 % decolorization of RB-224 being analyzed by UV?vis after 24 h of incubation. UV?vis spectroscopy and FTIR analysis have shown the efficiency of A. baumannii for degrading RB-224. In the UV spectrum, the shifting of the peak from560 nm to 400 nm confirmed the decolorization. In the FTIR spectrum, the shift of several significant peaks related to the functional groups S = O C-N, C?H, C = C, and N?H, corresponding to the structure of RB-224 dye along with the emergence of new peaks indicating the formation of metabolites after degradation. The Azoreductase (AZA) gene of ?600 bp was amplified and cloned in a pTz57R/T cloning vector. Subsequently, the AZA gene was sequenced and submitted to NCBI for accession number. This study explored the potential of A. baumannii isolate suitable for the bio-remediation of textile dyes. ? 2024 The Author(s)en_US
dc.description.natureFinalen_US
dc.identifier.ArtNo103279
dc.identifier.doi10.1016/j.jksus.2024.103279
dc.identifier.issue7
dc.identifier.scopus2-s2.0-85194905858
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85194905858&doi=10.1016%2fj.jksus.2024.103279&partnerID=40&md5=74d20bb6988ad81cb89496d93e203675
dc.identifier.urihttps://irepository.uniten.edu.my/handle/123456789/36449
dc.identifier.volume36
dc.publisherElsevier B.V.en_US
dc.relation.ispartofAll Open Access; Gold Open Access
dc.sourceScopus
dc.sourcetitleJournal of King Saud University - Science
dc.titleRole of Acinetobacter baumannii in decolorization of reactive blue 224 dye and functional analysis of azoreductase geneen_US
dc.typeArticleen_US
dspace.entity.typePublication
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