Publication: Extremely low frequency electromagnetic field generator suitable for plant in vitro studies
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Date
2017
Authors
Prihatini R.
Abdullah M.P.
Bin Tuan Abdullah T.A.R.
Said I.
Hussin H.
Saleh N.M.
Journal Title
Journal ISSN
Volume Title
Publisher
Czech Academy of Agricultural Sciences
Abstract
The extremely low frequency electromagnetic field (ELF-EMF) occurs naturally from the earth and artificially as a human invention. The objectives of this study were to develop a suitable ELF-EMF generator for in vitro plants culture studies and to determine the effect of ELF-EMF exposure on in vitro tobacco (Nicotiana tabacum) growth and chlorophyll content. An ELF-EMF generator, the coGEM 1, 000 was constructed using four coils of copper wires that were connected to a transformer, multimeter and rheostat. The coGEM 1, 000 suitable for tissue culture plants is able to produce stable and uniform 6 and 12 mT 50Hz ELF-EMF in the four coils of the ELF-EMF generator. The tobacco in vitro plantlets were exposed to 6 and 12 mT of 50 Hz ELF-EMF for a period of 0.5, 1, 2 and 4 hours. The exposure to 12 mT ELF-EMF for an hour increased plant growth (shoot height); whereas the exposure to 6 mT Elf-EMF for an hour increased chlorophyll a, chlorophyll b and the total chlorophyll content.