Publication: Structural and nanohardness evaluation of nanobiosensor materials
Date
2016
Authors
Alwinjit Singh Gurdip Singh
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Abstract
Enzyme immobilization can be characterized as a procedure of limiting the enzymes particles that is physically appended to a strong backing over which a substrate is passed and is then changed over to product. Then again, Immobilized Enzyme is characterized as enzymes whose development in space have been limited either totally or only a little locale.
Enzymes Immobilization has been chosen for the fact that immobilization eventually increases the enzymes stability since enzymes can easily depreciate in extreme climate for instance high temperature, pressure and inconsistence pH. This project has been conducted in order to study the structural characteristics of immobilized enzyme on porous nonporous materials besides to examine the nanohardness properties of the materials. The materials used for this project is mainly MOF-199 and SiO2
MOF-199 is a copper based metal natural structure which was likewise known as the Cu-BTC and HKUST-1. The MOF-199 materials fundamentally have a solitary crystalline octahedral morphology structure other than happening in nature with high surface area, high pore volume and the most critical low density while SiO2 consists of spherical morphology and nanoscale size.
The methods use for this project includes UV-VIS in order to observe the compounds that are present between before and after immobilization, FTIR in order to observe the formation of any new bonds between before and after immobilization, FESEM in order to observe the actual particles morphology between before and after immobilization, EDS in order to observe the atomic percentage between before and after immobilization and lastly nanoindentation testing in order to test the plastic deformation of the samples.
Description
T174.7.A48 2016
Keywords
Nanotechnology