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Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Production

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dc.citedby1
dc.contributor.authorZainal B.S.en_US
dc.contributor.authorZaini S.en_US
dc.contributor.authorZinatizadeh A.A.en_US
dc.contributor.authorMohd N.S.en_US
dc.contributor.authorIbrahim S.en_US
dc.contributor.authorKer P.J.en_US
dc.contributor.authorMohamed H.en_US
dc.contributor.authorid57200914760en_US
dc.contributor.authorid58114920300en_US
dc.contributor.authorid10139587200en_US
dc.contributor.authorid57192892703en_US
dc.contributor.authorid7202480735en_US
dc.contributor.authorid37461740800en_US
dc.contributor.authorid57136356100en_US
dc.date.accessioned2024-10-14T03:18:46Z
dc.date.available2024-10-14T03:18:46Z
dc.date.issued2023
dc.description.abstractA pre-culture stage is required to obtain robustly-dividing cells with high hydrogen (H2) production capabilities. However, a step-by-step process for biohydrogen production is scarcely reported, mainly from palm oil wastewater. Therefore, this study developed a guideline to find the best inoculum heat treatment conditions and implement the selected conditions for biohydrogen production using palm oil wastewater. This study used raw palm oil mill effluent (POME) and POME sludge as substrate and inoculum, respectively. Our findings reveal that 80 �C and 30 min were the best conditions for inoculum heat treatment. When testing the conditions on POME sludge and inoculating with raw POME (28 g COD/L) at 37 �C (reaction temperature), 24 h (reaction time), and pH 5.5, 34 mL H2/d was recorded. A slight increase (1.1-fold) was observed compared to 5 g COD/L POME co-digested with 5 g/L glucose (31 mL H2/d). This discovery indicates that raw POME is a potential source for biohydrogen production under anaerobic fermentation and can be directly used as substrate up to 30 g COD/L. The proposed guideline could also be implemented for different organic wastes for biohydrogen production study. � 2023 by the authors.en_US
dc.description.natureFinalen_US
dc.identifier.ArtNo127
dc.identifier.doi10.3390/fermentation9020127
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85148763601
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85148763601&doi=10.3390%2ffermentation9020127&partnerID=40&md5=ce42e52eafc0d752f86144eeccf1fd3d
dc.identifier.urihttps://irepository.uniten.edu.my/handle/123456789/34274
dc.identifier.volume9
dc.publisherMDPIen_US
dc.relation.ispartofAll Open Access
dc.relation.ispartofGold Open Access
dc.sourceScopus
dc.sourcetitleFermentation
dc.subjectanaerobic digestion
dc.subjectbiohydrogen
dc.subjectenergy
dc.subjectheat treatment
dc.subjectpalm oil
dc.subjectrenewable sources
dc.titlePreliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Productionen_US
dc.typeArticleen_US
dspace.entity.typePublication
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