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Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement

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dc.contributor.authorLim W.F.en_US
dc.contributor.authorAbdullah M.en_US
dc.contributor.authorHo K.L.en_US
dc.contributor.authorYap B.K.en_US
dc.contributor.authorLai M.I.en_US
dc.contributor.authorid50061393300en_US
dc.contributor.authorid55666914500en_US
dc.contributor.authorid18233493700en_US
dc.contributor.authorid26649255900en_US
dc.contributor.authorid13605357700en_US
dc.date.accessioned2023-05-29T08:07:06Z
dc.date.available2023-05-29T08:07:06Z
dc.date.issued2020
dc.description.abstractIntroduction: Iron deficiency anaemia (IDA) is the most common cause of anaemia worldwide. Determination of body iron status is necessary to diagnose IDA. This can be measured using a biochemistry assessment of the serum/ plasma. Plasma/serum iron quantitation is also important in diagnosing iron overload disorders. However, iron studies are limited due to high cost and lack of access to biochemical analysers. Therefore, a cost- and technical-effective method is needed to measure human plasma iron concentration. Plasma iron is mainly transferrin-bound and an acidic plasmic condition is necessary to release the iron. This study investigated various candidate acid salts to achieve the acidic condition needed for plasma iron release. Method: Ten powdered or crystallised acid salts were studied for their water solubility as well as their pH reduction capability in revised simulated body fluid (r-SBF) and commercially available human plasma without any change in colour or form. Results: Six acid salts studied were discontinued from further investigation because they were insoluble in water. Another two candidates were unsuitable as they precipitated in r-SBF and human plasma. Maleic acid formed a jelly-like texture after a certain amount of time in human plasma. Only citric acid met all the criteria of a suitable acid salt to be investigated further as part of the reagent for a spontaneous plasma iron measurement. Conclusion: Citric acid, which is a colourless and odourless acid salt, was selected to lower the human plasma pH to an acidic condition for transferrin-bound iron release. � 2020 UPM Press. All rights reserved.en_US
dc.description.natureFinalen_US
dc.identifier.epage57
dc.identifier.scopus2-s2.0-85099388878
dc.identifier.spage52
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85099388878&partnerID=40&md5=9aa54e9b0978a7ee3f13ff4f996c9c79
dc.identifier.urihttps://irepository.uniten.edu.my/handle/123456789/25170
dc.identifier.volume16
dc.publisherUniversiti Putra Malaysia Pressen_US
dc.sourceScopus
dc.sourcetitleMalaysian Journal of Medicine and Health Sciences
dc.titleSelection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurementen_US
dc.typeArticleen_US
dspace.entity.typePublication
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