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Enhancement of lipase enzyme activity in non-aqueous media through a rapid three phase partitioning and microwave irradiation

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dc.citedby24
dc.contributor.authorSaifuddin N.en_US
dc.contributor.authorRaziah A.Z.en_US
dc.contributor.authorid22135844300en_US
dc.contributor.authorid25825363100en_US
dc.date.accessioned2023-12-29T07:56:31Z
dc.date.available2023-12-29T07:56:31Z
dc.date.issued2008
dc.description.abstractThree phase partitioning is fast developing as a novel bio-separation strategy with a wide range of applications including enzyme stability and enhancement of its catalytic activity. pH tuning of enzyme is now well known for use in non-aqueous systems. Tuned enzyme was prepared using a rapid drying technique of microwave dehydration (time required around 15 minutes). Further enhancement was achieved by three phase partitioning (TPP) method. With optimal condition of ammonium sulphate and t-butanol, the protein appeared as an interfacial precipitate between upper t-butanol and lower aqueous phases. In this study we report the results on the lipase which has been subjected to pH tuning and TPP, which clearly indicate the remarkable increase in the initial rate of transesterification by 3.8 times. Microwave irradiation was found to increase the initial reaction rates by further 1.6 times, hence giving a combined increase in activity of about 5.4 times. Hence it is shown that microwave irradiation can be used in conjunction with other strategies (like pH tuning and TPP) for enhancing initial reaction rates.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.1155/2008/920408
dc.identifier.epage871
dc.identifier.issue4
dc.identifier.scopus2-s2.0-57349185653
dc.identifier.spage864
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-57349185653&doi=10.1155%2f2008%2f920408&partnerID=40&md5=d55d8f3ddbf98c35e077c86ec2467cc6
dc.identifier.urihttps://irepository.uniten.edu.my/handle/123456789/30954
dc.identifier.volume5
dc.pagecount7
dc.publisherWWW Publications PTE Indiaen_US
dc.relation.ispartofAll Open Access; Gold Open Access; Green Open Access
dc.sourceScopus
dc.sourcetitleE-Journal of Chemistry
dc.subjectEnzyme in organic media
dc.subjectLipase-Candida rugosa
dc.subjectMicrowave drying
dc.subjectMicrowave irradiation
dc.subjectpH tuned enzyme
dc.subjectThree phase partitioning
dc.titleEnhancement of lipase enzyme activity in non-aqueous media through a rapid three phase partitioning and microwave irradiationen_US
dc.typeArticleen_US
dspace.entity.typePublication
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